polyclonal rabbit anti phospho nf κb p65  (Cell Signaling Technology Inc)

Journal: Frontiers in Immunology

Article Title: Extracellular microvesicles from patients with Rheumatoid arthritis promote dendritic cell activation in vitro

doi: 10.3389/fimmu.2025.1532114

Figure Lengend Snippet: Analysis of MAPK and NF-κB activation in DCs stimulated with RA patient EMVs. DCs untreated or stimulated with LPS, RA patient EMVs and HD EMVs were lysed and analyzed by western blot to evaluate: (A) Phospho-p38 expression using rabbit anti-phospho-p38 Ab. (B) Phospho-ERK expression using rabbit anti-phospho-ERK1/2 Ab. (C) phospho-NF-κB-p65 expression using rabbit anti-phospho-NF-κB-p65 Ab. Anti-β-tubulin mAb or anti-β-actin mAb were used to evaluate loading controls. Samples from one representative RA patient and HD are shown. Densitometric phospho-proteins/total proteins ratios were calculated in all HDs and RA patients and are summarized by histograms in the right panels of the figure. Data are reported as mean (S.D.). Statistical analysis indicated: **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The PVDF membranes containing the samples stimulated for 15 min were incubated with polyclonal rabbit anti-phospho-ERK1/2 or anti-phospho-p38 Abs (Cell Signaling, Inc. Danvers, MA, USA); alternatively, the PVDF membranes containing samples stimulated for 45 min were incubated with polyclonal rabbit anti-phospho NF-κB-p65 (Cell Signaling, Inc.).

Techniques: Activation Assay, Western Blot, Expressing